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Literature summary extracted from

  • Nakahata, K.; Matsunami, K.; Kobayashi, C.; Omori, T.; Xu, H.; Firdawes, S.; Fukuzawa, M.; Miyagawa, S.
    Analysis of the serine protease function of porcine factor I produced by liver cells for xenotransplantation (2008), Transpl. Immunol., 19, 30-36.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.45 medicine the serine protease domain of pig factor I indicates that the serine protease functions not only in pig serum, but in human and other serum, as well. Therefore, pig factor I may be considered a substitute for human factor I for the function of serine protease during the treatment of hepatic failure Sus scrofa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.45 serine protease region of factor I flanked by the Xho I site and cloned into vector pCXN2L/FLAG-PI. Plasmid transformed into Escherichia coli C600 and amplified. Expression in CHO cells Sus scrofa
3.4.21.45 serine protease region of factor I flanked by the Xho I site and cloned into vector pCXN2L/FLAG-PI. Plasmid transformed into Escherichia coli C600 and amplified. Expression in CHO cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.45 membrane surface-bound form of the serine protease domain Sus scrofa 16020
-
3.4.21.45 membrane surface-bound form of the serine protease domain Homo sapiens 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.45 Homo sapiens P05156
-
-
3.4.21.45 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.45 glycoprotein N-glycosylation sites are Asn-73, 421, 493 and 535, and a O-glycosylation sites is Thr-36. Has little species-specificity like human factor I, according to the relatively high homology of the AA sequences in the serine protease region, in comparison with those of membrane complement regulatory proteins Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.45 liver
-
 Sus scrofa
-
3.4.21.45 plasma
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.45 additional information can be expressed on xenogeneic cell membranes Sus scrofa ?
-
 ?
3.4.21.45 additional information human factor I has little species-specificity like pig factor I, according to the relatively high homology of the AA sequences in the serine protease region, in comparison with those of membrane complement regulatory proteins Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.45 factor I
-
 Sus scrofa
3.4.21.45 factor I
-
 Homo sapiens